Alamo Peptides

Sample Information

Product

Retatrutide

Marketing Name

AP-RT

Lot / Batch

AP-RT20-0926

Labeled Size

20 mg

Mol. Formula

C221H342N46O68

CAS Number

2381089-83-2

Cap Color

Green

Crimp Color

Silver

Identity Confirmation (LC-MS)

Pass

LC-MS (ESI+)

Reference Standard

Confirmed match to labeled compound

Identity

Confirmed

Heavy Metal Analysis

Pass

ICP-OES, USP <232>/<233>

Reference Standard

Within USP <232>/<233> elemental impurity limits

Purity Analysis (HPLC/UV)

Pass

HPLC with UV-Vis Detection

Reference Standard

>98%

Purity

99.5%

Endotoxin Analysis

Pass

Kinetic Chromogenic LAL, USP <85>

Reference Standard

Not detected above 5 EU/mL (USP <85>)

Fentanyl Presence Analysis

Pass

Targeted LC-MS/MS Screening

Reference Standard

Not detected

Net Content / Quantity Check

Pass

Gravimetric + HPLC Quantitation

Reference Standard

20mg ± 10%

Net Content

20.453 mg

Rapid Sterility Screen

Pass

Membrane Filtration + Chromogenic Detection

Reference Standard

No microbial growth observed

Conformity Vial (Batch Verification)

Pass

HPLC Comparison to Reference Vial

Purity

99.5%

Net Content

20.453 mg

Vial	Purity (%)	Net Content (mg)
Vial 1 (submitted)	99.38	20.54
Vial 2 (conformity)	99.58	20.13
Vial 3 (conformity)	99.54	20.69

Purity, batch mean

99.50%

SD 0.086 · n=3

Net content, batch mean

20.45 mg

SD 0.237 · n=3

Sample Photo

Methods & Notes

• The sample was confirmed to be Retatrutide by LC-MS (ESI+). Identification by comparison of observed mass against the expected molecular weight.
• Elemental impurities quantified by ICP-OES per general principles of USP <232>/<233>. Screened elements include lead, mercury, arsenic, and cadmium.
• Peptide purity determined by reversed-phase HPLC with UV detection at 214 nm. Value represents the percentage of the target compound relative to all detected compound-related peaks.
• Additional vials from the same lot and concentration were compared against the primary tested vial by HPLC to confirm consistency across the production run. See Batch Conformity Results below for per-vial figures.
• Bacterial endotoxin quantified by kinetic chromogenic LAL per general principles of USP <85>. Result reported in EU/mL against the defined acceptance limit for the sample type.
• Targeted screening for fentanyl and common fentanyl analogs performed by LC-MS/MS. Result reported as detected or not detected against an established cutoff threshold.
• Net content determined gravimetrically and cross-referenced against HPLC peak area quantitation relative to a characterized reference standard.
• Preliminary microbial contamination screening performed by membrane filtration with chromogenic detection; results are read after 24 to 48 hours of incubation. This screen is a fast preliminary indicator and is not a substitute for full compendial sterility testing.

Sample identifications, product names, and lot numbers are provided by the submitting party and are not independently verified. Results reflect only the specific sample submitted and are not an evaluation of the manufacturer's processes or overall production. PurePoint Analytical is an independent analytical testing laboratory.